Grodno, 7 Antoni Tyzenhauz Square
+375152559075
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INSTITUTE OF BIOCHEMISTRY OF BIOLOGICALLY ACTIVE COMPOUNDS

Laboratory for monitoring the micronutrient status of the population

APPLIED RESEARCH LABORATORY FOR MONITORING OF NUTRITIONAL (MICRONUTRIENT) STATUS OF THE POPULATION AND DEVELOPMENT OF TECHNOLOGIES FOR ITS CORRECTION USING FUNCTIONAL PRODUCTS AND BIOLOGICALLY ACTIVITE ADDITIVES

Phone: +375152559079

 

Main activities:

  • Monitoring of micronutrient status in children of different ages.
  • Assessment of the condition and correction of microelement status in the population of the regions affected by the Chernobyl accident.
  • Control of the content of toxic elements in food and food raw materials.
  • Study of microelement components in soil and water sources of the Grodno region.

 

Industry laboratory services

  1. Development of the composition and formulation of food additives for the creation of functional foods, biologically active additives .
  2. Preclinical biological testing of dietary supplements, medicines, food supplements, etc. on cell culture and on experimental models on laboratory animals.
  3. Testing of antidiabetic drugs on β-cell culture, etc.
  4. In vitro testing of drugs that reduce the degree of protein glycosylation.
  5. Testing the cytotoxicity of chemotherapy drugs on cell cultures.
  6. Study of the microelement composition of water, soil, biological material (blood, urine, tissues of animals, plants and humans), dietary supplements, pharmaceutical substances; perhaps, the study of the trace element composition of minerals (the method needs to be improved).

 

Laboratory equipment

  • Liquid chromatograph «Agilent Technologies»
  • Gas chromatograph «Agilent Technologies»
  • NexION2000B Inductively Coupled Plasma Mass Spectrometer

 

Experimental Models

  • models of diabetes 1 (alloxan, streptozotocin) and type 2 diabetes (high-fat diet + streptozotocin in low doses);
  • models of acute and chronic stress: hypokinesia, hypoxia, psycho-emotional stress, audiogenic stress, physical activity (running), combined stress;
  • hypothyroidism model (methimazole-induced, bilateral and unilateral thyroidectomy), iodine deficiency model and excess iodine intake model;
  • model of exposure to acute (small, medium, large doses) and chronic ionizing radiation (small, medium doses);
  • model of ischemia and ischemia/reperfusion of the kidneys;
  • immunodeficiency model;
  • pantothenic deficiency model.

 

Research methods practiced in the laboratory

  • determination of the concentration of fat-soluble vitamins by HPLC: A, E, D - in blood serum and animal tissues, food additives, food products, dietary supplements, pharmaceutical substances, etc.;
  • determination of the concentration of water-soluble vitamins: - vitamin C (urine and blood), pyridoxine (in the blood);
  • riboflavin, thiamine, pyridoxylic acid (in urine) and in other biological materials (blood serum, tissues), as well as milk, food additives, foodstuffs, pharmaceutical substances, dietary supplements, etc.;
  • determination of the concentration of hormones by HPLC: - corticosterone, aldosterone, testosterone in blood serum;
  • determination of the concentration of coenzyme A and acetyl-CoA by HPLC in animal and human tissues in dietary supplements;
  • determination of the concentration of microelements by mass spectrometry with inductively coupled plasma: - water, urine, tissues, food additives, foodstuffs, dietary supplements, etc., animals and humans;
  • determination of indicators of the antioxidant system by the spectrophotometric method: - SOD, catalase, GPO, GR, GT; determination of the total antioxidant activity of serum and tissues;
  • determination of the concentration of low molecular weight antioxidants (glutathione), as well as the concentration of non-protein, protein thiols and their total in serum and tissues;
  • determination of the concentration of LPO indicators: - concentration of TBRS, as well as DPA-reactive products;
  • determination of the concentration of free fatty acids in the blood and tissues by gas chromatography.

 

Projects and tasks carried out in the laboratory

  • Task 4.1.5 "Vitamin and microelement status in metabolic disorders and its correction with natural immunomodulators, microelements and vitamin derivatives";
  • Agreement No. 12-10/21/02 "Study of the effectiveness of samples of compositions based on natural plant raw materials that improve memory."
  • Agreement 14/02-22 "Study of the stress-protective effects of samples of compositions based on natural plant materials in rats under the influence of combined stress (hypokinesia + psycho-emotional stress)";
  • BRFFR grant No. M21ARM-019 "Chemical synthesis of substrates and development of a new method for determining the activity of pantetheinase in biological fluids for the purpose of early laboratory diagnosis of acute renal failure."

 

List of publications

  1. Maksimchik Yu. Z., Khvesko I. S., Ostrovsky A. A., Moiseyonok A. G. Epigenetic factors in metabolism and mechanisms of vitamin D activity // Biochemistry and molecular biology / Biochemistry and molecular biology. Issue 2. Boundaries of biological sciences. Signaling and Metabolism (Frontiers in Life Science. Signaling and Metabolism): Proceedings of the 1st Belarusian-Polish-Lithuanian scientific conference dedicated to the 250th anniversary of Academician A. Snyadetsky, Grodno, November 8–9, 2018. - Minsk: Information Center of the Ministry of Finance, 2018. - S. 99–104.
  2. Bashun N.Z., Gurinovich V.A., Anufrik S.S., Chekel A.V., Maksimchik Yu.Z., Filipovich N.A., Maksimovich V.A. Features of the micronutrient status of students with different levels of physical activity and body mass index // News of Biomedical Sciences. News of biomedical sciences. - Minsk, 2018. - T. 18. - No. 3. - P. 12-13.
  3. Raduta E.F., Lukienko E.P., Maksimchik Yu.Z., Filipovich N.A., Semenovich D.S., Moiseenok A.G. Correction of disorders of the prooxidant-antioxidant balance caused by cerebral ischemia under the action of metabolic therapy // Modern problems of biochemistry and molecular biology: coll. Art. II Belarusian Biochemical Congress, Grodno, May 17–18, 2018 / National Academy of Sciences of Belarus; RNIUP "Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus"; under total ed. I.N. Semeneni, A.G. Moiseenka. - Minsk: Information Center of the Ministry of Finance, 2018. - S. 487–491.
  4. Kanunnikova N.P., Semenovich D.S., Gurinovich V.A., Lukienko E.P., Titko O.V., Mamchits D.K., Pesnyak A.V., Moiseenok A.G. Neurochemical effects of modulation of the CoA system in aluminum neurotoxicosis / Biochemistry and molecular biology: collection of scientific papers, issue No. 3; ch. ed. I.N. Semenya. - Minsk: Information Center of the Ministry of Finance, 2019. - S. 95-98.
  5. Gurinovich V.A., Khvesko I.S., Maksimchik Yu.Z., Moiseyonok A.G. Potentiation of the bioavailability of the micellar form of cholecalciferol with the combined use of vitamins A and K / Biochemistry and molecular biology: collection of scientific papers, issue No. 3; ch. ed. I.N. Semenya. - Minsk: Information Center of the Ministry of Finance, 2019. - S. 109-111.
  6. Maksimchik Yu.Z., Khvesko I.S., Gurinovich V.A., Ostrovsky A.A., Moiseenok A.G. Tolerant level of consumption, hypervitaminosis and vitamin D toxicity // Nutrition and metabolism: collection of scientific articles, issue No. 5; scientific ed. A.G. Moiseyonok. - Minsk: Belarusian Science, 2020. - P.130–139
  7. Maksimchik Yu.Z., Rozhko V.A., Cheremisin A.S., Filipovich N.A., Lukienko E.P., Yarets Yu.I., Semenenya I.N. Assessment of microelement status in blood plasma in patients with autoimmune thyroiditis // Biochemistry and molecular biology: collection of articles, issue No. 4. / ch. ed. I.N. Semenya. - Minsk: Information Center of the Ministry of Finance, 2020. - P. 126–131.
  8. Moiseyonok A.G., Kanunnikova N.P., Gurinovich V.A., Lukienko E.P., Maksimchik Yu.Z., Katkovskaya I.N., Khvesko I.S. Prospects for studying the formation and correction of the redox status of CNS structures by means of redox-active forms of calciferols and pantothenic acid // Biologically active substances of natural origin in the regulation of vital processes: a collection of articles of the Intern. scientific-practical. conf. dedicated to the 50th anniversary of the Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus (Grodno, October 5–6, 2021). - Minsk: Information Center of the Ministry of Finance, 2021. - S. 301-304.
  9. Lukienko E.P., Titko O.V., Katkovskaya I.N., Murashkevich Yu.G., Kanunnikova N.P. Effects of derivatives of vitamins, microelements and lactoferrin on the indicators of redox balance in the blood plasma of rats with experimental diabetes // Collection of materials of the Republican scientific and practical conference with international participation, dedicated to the 85th anniversary of the birth of Professor Borisyuk Mikhail Vladimirovich (February 17, 2022. ) / editorial board: V.V. Vorobyov (responsible editor), V.V. Zinchuk, V.N. Khilmanovich. - Grodno: GrGMU, - S. 162-164.
  10. Nadolnik L.I., Katkovskaya I.N., Titko O.V., Murashkevich Yu.G., Maksimchik Yu.Z., Filipovich N.A., Lukienko E.P., Cheremisin A.S., Khvesko I. .S., Polubok V.Ch., Shuriberko A.V., Gurinovich V.A. Indicators of the antioxidant system and lipid peroxidation in the kidneys of rats when modeling ischemia and ischemia/reperfusion // Oxygen and free radicals: collection of materials. scientific-practical. conferences with international participation, May 26-27, 2022 / ed. prof. V.V. Zinchuk. - Grodno: GrGMU, 2022. - S. 121-124.