Grodno, 7 Antoni Tyzenhauz Square
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Sectors

Sector of Molecular Biology

The sector was established in 2019 on the basis of the department of medical and biological problems of alcoholism.

Shuriberko Alexey Vladimirovich

Position: manager

Phone: +375336867892

E-mail: shuriberko@ibiochemistry.by

Profile in eLibrary

 

The main directions of scientific research of the sector:

  • fundamental and applied research in biochemistry and molecular biology;
  • development of modern technologies for diagnosing regimens, duration and prescription of alcohol consumption using biochemical markers to solve the problems of expert and medical practice;
  • study of the mechanisms of influence of physical factors on biochemical processes in the body in normal and pathological conditions;
  • screening of substances of natural origin as promising means for the treatment of metabolic violation;
  • studies on animal cell cultures (release, cultivation, directed differentiation of stem cells, systematic assessment of the biological properties of medicinal substances);
  • molecular genetic studies (expression, gene polymorphisms, PCR diagnostics).

 

Methodological possibilities

  • spectrophotometric and spectrofluorimetric methods;
  • analysis of activity of a wide range of enzymes, concentration of substrates, analysis of antioxidant protection;
  • bioenergy research;
  • polymerase chain reaction (PCR) studies of gene expression and Western blot analysis of proteins;
  • performing ELISA studies;
  • research and testing on cell cultures;
  • HPLC, chromato-mass spectrometry.

 

Bioenergetics and mitochondrial parameters

  • isolation of MCh by methods of differential centrifugation, or in Percoll density gradients;
  • determination of mitochondrial respiration parameters on a polarograph (basal respiration, substrate respiration, ADP-stimulated, uncoupled, in the presence of pore inhibitors, determination of respiratory control, ADP/O);
  • measuring the rate of ROS/peroxide production by the method with AmplexRed;
  • determination of the membrane potential of mitochondria in various metabolic states;
  • measuring the functioning of the MPTP pore;
  • measurement of the activity of MX enzymes of the TCA (succinate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, aconitase-2, 2-oxoglutarate dehydrogenase);
  • study of the functioning of electron transport chain complexes (I-V complexes);
  • study of the antioxidant system МХ (SH-groups, glutathione peroxidase, -reductase, catalase, products of lipid peroxidation and oxidative modification of proteins, SOD-Mn, SOD-Cu/Zn);
  • semi-quantitative assessment of mitochondrial DNA damage.

 

Material and technical equipment:

  • PCR laboratory

   

  • cell culture laboratory

   

  • biochemical laboratory